Fig. 5.

H3.3 supports TF binding during differentiation. A Pairwise comparison of TF activity at promoters between WT ESCs and EBs. TF motifs enriched in EBs have negative differential binding scores and TF motifs enriched in ESCs have positive differential binding scores. B Pairwise comparison of TF activity at promoters between WT and H3.3 KO EBs. TF motifs enriched in WT EBs have negative differential binding scores and TF motifs enriched in H3.3 KO EBs have positive differential binding scores. For both panels, the volcano plot shows differential binding activity against the −log10(p-value) for all investigated TF motifs. Each TF is represented by a single circle (n = 458). Representative differentiation-specific TFs are labeled in purple and representative pluripotency-specific TFs are labeled in green