Fig. 3.

Chromatin landscape is dysregulated with H3.3 loss. A Average profiles (top) and heatmaps (bottom) of ATAC-seq, H3K4me3, p300, H3K27ac, and BRD4 enrichment at active promoters (n = 12,903) in WT and H3.3 KO ESCs. Three kilobases around the center of promoters are displayed for each analysis. B–E Boxplots showing B H3K4me3, C p300, D H3K27ac, and E BRD4 enrichment at active promoters in WT and H3.3 KO ESCs (n = 12,903). The bottom and top of the boxes correspond to the 25th and 75th percentiles, and the internal band is the 50th percentile (median). The plot whiskers correspond to 1.5× interquartile range and outliers are excluded. p-values determined by Wilcoxon rank sum two-side test. F Ratio (log2) of H3K4me3, p300, H3K27ac, and BRD4 enrichment at active promoters (n = 12,903) in WT and H3.3 KO ESCs. x-axis values <0 indicate reduced enrichment in the absence of H3.3