Fig. 1.

Loss of H3.3 reduces chromatin accessibility at promoters. A Correlation plot between ATAC-seq and H3.3 ChIP-seq at active promoters (n = 12,903) in ESCs. B Genome browser representations of ATAC-seq in WT and H3.3 KO ESCs and WT and HIRA KO ESCs. The y-axis represents read density in reads per kilobase per million mapped reads (RPKM). C ATAC-seq average profiles at active promoters (n = 12,903) in WT and H3.3 KO ESCs. D Correlation plot between differential ATAC-seq signal in H3.3 KO compared to WT ESCs and H3.3 enrichment at active promoters (n = 12,903) in WT ESCs. E Boxplot showing ATAC-seq signal at active promoters and promoters binned by H3.3 enrichment in WT and H3.3 KO ESCs. The bottom and top of the boxes correspond to the 25th and 75th percentiles, and the internal band is the 50th percentile (median). The plot whiskers correspond to 1.5× interquartile range and outliers are excluded. p-values determined by Wilcoxon rank sum two-side test