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Fig. 1 | Genome Biology

Fig. 1

From: DNA methylation entropy as a measure of stem cell replication and aging

Fig. 1

Methylation changes in stem cells correlate highly with their progeny. a Schematic diagram of experimental procedures. Mice were sacrificed at 4, 12, 18, or 24 months and intestines separated into the upper small intestine (USI), lower small intestine (LSI), and colon (COL). Stem cells were separated by sorting for Lgr5-GFP + cells. Reduced representation bisulfite sequencing (RRBS) and RNA-seq were done on the samples. b Principal component analysis (PCA) of 180,496 CpG sites common across 4 age groups and 3 tissues. Colon samples form a different cluster than small intestinal samples (PC1), and the spread along PC2 corresponds to an increasing age. c Volcano plots showing methylation differences between stem cells (Lgr5 − GFP +) and nonstem cells (Lgr5 − GFP −) in young and old mouse colon samples. Each volcano plot indicates the number and percent of CpG sites that are differentially methylated (Methylation change >  ± 5%, q-value < 0.05). d Volcano plots showing methylation differences between young (4 months) and old (24 months) samples in stem cells and nonstem cells. e Scatterplot of CpG sites that change significantly between 4 months versus 24 months in either stem cells, nonstem cells, or both in COL, USI, and LSI

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