Fig. 1

YTHDF1 and YTHDF2 have different molecular functions. a UpSet plots comparing transcripts analyzed with m⁶A-centric and RBP target-centric approaches. b Venn diagram showing RNA analyzed by Zaccara et al. and RNA analyzed in this work. c Cumulative plots showing changes in RNA translation efficiency (TE, left) and abundance (right) after YTHDF1 knockdown using data from Zaccara et al. P values were determined by a Mann-Whitney-Wilcoxon test. d Cumulative plots showing changes in RNA translation efficiency (TE, left) and abundance (right) after YTHDF2 knockdown using data from Zaccara et al. P values were determined by a Mann-Whitney-Wilcoxon test. e Sequence alignment of YTHDF proteins showing differences in the low complexity domain (LCD) region. Conservation scores were calculated by Jalview [22]. f Heatmaps showing the similarity scores calculated by the BLOSUM62 algorithm of YTHDF LCDs (left) and full-length proteins (right). g Higher-order structures formed by LCDs of YTHDFs under an electron microscopy (EM). Scale bar: 200 nm. h Cytoplasmic RNP granule protein localization map of the cell generated by t-distributed stochastic neighbor embedding (t-SNE) from the CELL MAP project [23]. YTHDF1 and eIFs are delineated in red, YTHDF2 and CNOTs are delineated in blue, and YTHDF3 is delineated in yellow. For boxplots, the center line represents the median, the box limits show the upper and lower quartiles, and whiskers represent 1–99%. P values were determined by a Mann-Whitney-Wilcoxon test