Fig. 2From: Leveraging massively parallel reporter assays for evolutionary questionsOverview of MPRA data and analysis. For both A barcoded MPRA and B STARR-seq experiments, pre-transfection (or pre-infection) plasmid-derived DNA is sequenced to control for variation in the pool of fragments input into the experiment, while post-experient plasmid-derived RNA is sequenced to measure regulatory output. In both cases, the post-experiment mRNA-to-DNA ratio therefore reflects regulatory activity controlling for variation in input. In A barcoded MPRAs, the sequencing target is the fragment-associated barcode, while in B STARR-seq experiments, the sequencing target is the query fragment itselfBack to article page