Fig. 6

Multiple gene targeting and adeno-associated virus (AAV) delivery of CCRS in cancer cells. a A schematic diagram of CCRS delivered by AAV, which effectively inhibits tumors by targeting multiple oncogenes. DR, direct repeat. fgRNA, fusion guide RNA. b Growth curves of bladder cancer T24 cells treated with CCRS and other existing technologies. The curves of cell proliferation were compared using analysis of variance (ANOVA). The results at each time point are shown as the mean ± SD. Each experiment was independently performed in triplicate five independent times. Each error bar indicates the variation between the means of five independent experiments. c AAV-CCRS efficiently inhibited in vivo tumor growth. The luminescent signal intensities for AAV-CCRS, AAV-CasRx and AAV-shRNA and their corresponding negative controls are shown. Data are shown as the mean ± SD. **p < 0.01, between the groups, using a two-tailed t-test. d Quantification for bioluminescence imaging of a metastatic model. The luminescent signal intensities for AAV-CCRS, AAV-CasRx and AAV-shRNA are shown. Data are shown as the mean ± SD. **p < 0.01, between the groups, using a two-tailed t-test