Fig. 5

Multiplexed targeting of diverse human coding and non-coding transcripts with CCRS. a Multiple fusion guide RNAs targeting diverse target transcripts can be expressed as a single array and processed by CasRx protein into individual guide RNAs within the same cell. b qRT-PCR analysis of relative target RNA knockdown by CCRS-array, CasRx crRNA-array, and shRNA-array. Repression efficiency values were the decline percentages (%) of transcript expression levels relative to the corresponding negative control. Data are expressed as the mean ± SD. Each experiment was performed in triplicate five independent times. Each error bar indicates the variation between the means of five independent experiments. *p value < 0.05, and **p value < 0.01, relative to the control using a two-tailed t-test