Fig. 3

Targeting endogenous transcripts with CCRS. a Knockdown of six endogenous transcripts following transfection of cells with CCRS and other existing technologies was assayed using qRT-PCR. b Knockdown of six endogenous transcripts using CCRS, CCRS-NLS, and CCRS-NES. NLS, nuclear localization signal. NES, nuclear export signal. Repression efficiency values are the decline percentages (%) of the transcript expression levels relative to the corresponding negative control. The results are shown as the mean ± SD. Each experiment was performed in triplicate five independent times. Each error bar indicates the variation between the means of five independent experiments. *p value < 0.05, and **p value < 0.01, relative to the control using a two-tailed t-test