Fig. 7From: Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activationAnalysis of oncogene hKRASG12D expression and tumor suppressor (TP53, LKB1) mutation in DIC pig with metastatic pancreatic adenocarcinoma. a Q-PCR analysis of hKRASG12D expression levels in six pancreatic tumor samples normalized to control samples from wild-type pigs. Means of three biological replicates were shown. Error bars indicate SEMs. b Indel frequency analysis by amplicon deep sequencing of TP53-gRNA or LKB1-gRNA targeting sites in the pancreas and other metastatic organs from DIC pig injected with AAV6-PKL. Data are plotted as mean ± SEM and each dot represents a different tissue section. c–d Mean proportions related to varied indel size for LKB1-gRNA targeting site (c) or TP53-gRNA targeting site (d) in the pancreas and other metastatic organs from DIC pig injected with AAV6-PKL. e–f Distribution of indel for LKB1-gRNA targeting site (e) or TP53-gRNA targeting site (f) in the pancreas and other metastatic organs from DIC pig injected with AAV6-PKL. Dots of one color represent one different tissue section. g Heatmap analysis for simultaneous length distribution and mean proportion of indel in LKB1 and TP53 loci, respectively, in the pancreas and other metastatic organs from DIC pig injected with AAV6-PKLBack to article page