Skip to main content
Fig. 5 | Genome Biology

Fig. 5

From: Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation

Fig. 5

Effective in vivo genome editing and metastatic pancreatic adenocarcinoma modeling. a Schematic representation of the generation of pancreatic adenocarcinoma models with TP53−/−; KRASG12D; LKB1−/− (PKL). b Injection of AAV6-PKL or 0.9% saline into the main pancreatic duct or the body of the pancreas. c Indel frequency measured by amplicon deep sequencing of TP53-gRNA (n = 5), P = 0.0109, *P < 0.05 or LKB1-gRNA (n = 10), P = 9.13×10−6, ****P < 0.0001. Unpaired, two-tailed t-test was used for statistical analyses. d–e Classification of amplicon deep sequencing of pancreas tissue sections from DIC-F1-82-P14 (d) and DIC-F1-91-P10 (e) at pLBK1 and pTP53 loci, respectively. The representative top 5 indel reads were shown. f Post about 21-week AAV injection, PET-CT imaging for AAV6-PKL-injected DIC pig (DIC-F1-96). g Pictures of multiple organs from the abdominopelvic cavity of AAV6-PKL-injected DIC pig. Hyperplastic and adherent neoplasms were found in the liver, large intestine, diaphragm, and small intestine, and lymph nodes were enlarged

Back to article page