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Fig. 4 | Genome Biology

Fig. 4

From: Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches

Fig. 4

Venn diagrams showing the number of predicted off-target sites for sgRNAs targeting a human PCSK9, b human Albumin, c mouse PCSK9, d mouse Albumin, e human FANCF, f human VEGFA, and g human HBB, determined by the indicated methods. Validation indicates targets validated by targeted deep sequencing. Red* represents the number of off-target sites that were confirmed manually (Additional File 3: Table S2). h Miss rates of Extru-seq and GUIDE-seq determined by considering all of the off-target sites that underwent validation experiments for sgRNAs targeting human PCSK9, human Albumin, mouse PCSK9, mouse Albumin, human FANCF, human VEGFA, and human HBB in HeLa cells. The horizontal lines represent the mean (*P < 0.05 in two-sided unpaired Mann-Whitney test). i Off-target sites missed by GUIDE-seq and the distribution of the number of mismatches (indicated in parentheses). Deep blue (0), orange (1), gray (2), yellow (3), sky blue (4), green (5), and navy (6)

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