Fig. 3

Venn diagrams showing the number of predicted off-target sites for sgRNAs targeting a human FANCF, b human VEGFA, and c human HBB, determined by the indicated methods. d Validation rates of the off-target sites predicted by DIG-seq, Digenome-seq, Extru-seq, and GUIDE-seq for sgRNAs targeting the FANCF, VEGFA, and HBB genes in HeLa cells. The horizontal lines represent the mean (ns, no significance in two-sided unpaired Mann-Whitney test). e p-values obtained by the normalized rank sum test for each pair of off-target prediction methods for sgRNAs targeting PCSK9 and Albumin in human and mouse cells in addition to FANCF, VEGFA, and HBB in HeLa cells. The horizontal lines represent the mean. (n ≥ 16 were selected to be analyzed.) Dotted line represents p = 0.05