Fig. 2

Venn diagrams showing the number of predicted off-target sites for sgRNAs targeting a human PCSK9, b human Albumin, c mouse PCSK9, and d mouse Albumin, determined by the indicated methods. e Validation rates of the top off-target sites predicted by an in silico method, GUIDE-seq, Digenome-seq, and Extru-seq in human and mouse cells for promiscuous sgRNAs targeting the PCSK9 and Albumin genes. The horizontal lines represent the mean (*P < 0.05, ns, no significance in two-sided unpaired Mann-Whitney test)