Fig. 1

Overview of seeding construction. A Open syncmers are constructed from the sequence, and randstrobes are constructed by linking together syncmers. The second strobe is sampled with a minimum and maximum spread between \([w_{min}, w_{max}]\) strobes downstream from the first strobe. While syncmers may occur several times due to repeats on the genome (red and green boxes), randstrobes are less repetitive. B Two reads, \(r_1\) and \(r_2\), are mapped to the reference. Finding matches using only syncmers creates several candidate locations, while the randstrobes are unique in this scenario. In the illustration, the syncmers are spread out for visual purposes. Panel C shows a real sampling of randstrobes on a DNA sequence and their spread using (2, 20, 4, 11) with sampling skew