Fig. 8

Cellular origin of response and non-response genes to cancer immunotherapy. A Differential expression analysis between responders (complete and partial responders, n = 12) and non-responders (stable and progressive disease, n = 33) performed on bulk RNA-seq from a total of 45 gastric cancer patients that underwent immunotherapy. Genes for downstream analysis (red) with adjusted p-value < 0.1 and log2 fold change > 0.25. B Up- and downregulated genes (A) were clustered based on their expression in minor cell types of the single-cell RNA-seq data (Additional file 1: Fig. S3). Here, a heatmap of the scaled average expression from the upregulated gene clusters is shown per major cell types of the single-cell RNA-seq on gastric cancer. C,D Annotation and UCell score of myeloid cells with gene cluster R8 (C) and fibroblasts with gene clusters NR10-13 (D) visualized on UMAP. E ROC curves of gene signature scores corresponding with specific cell subtypes on response data from cohort in A