Fig. 6

Cell communication inferred from single-cell transcriptome profiles in gastric cancer show a central role of F13-Activated-CTHRC1 fibroblasts. A Closeness centrality ranking of all cell subtypes in the inferred cluster-wise cell communication network, top 10 subtypes are shown. B Communication strength of F13-Activated-CTHRC1 fibroblasts with other cell subtypes, only significant communication (connectivity > 0.05) are drawn. C Significant ligand-receptor pairs in F13-Myeloid communication highlight tumor-enriched activation of integrin-Akt pathway in myeloid cells by F13-CTHRC1 cells. D Significant ligand-receptor pairs in F13-Endothelial communication show a tumor-restricted program for intercellular communication. The X-axis represent ligand and receptor pairs, with the first gene expressed on F13-Act-CTHRC1 cells and second gene expressed on the interacting cell types denoted in the Y-axis. Red: upregulated gene in tumor bulk RNA-seq. Blue: downregulated in tumor bulk (none present). E Communication strength of F13-CTHRC1 with other cell subtypes, red line denotes the cluster-wise connectivity cutoff of 0.05 (see “Methods”)