Fig. 1

Target-capture bisulfite sequencing in 807 GTEx samples confirms systemic interindividual epigenetic variation at CoRSIVs. A DNA samples were obtained from multiple tissues (representing the three embryonic germ layers) from each of 188 GTEx donors. B CoRSIV capture process using Agilent reagents. C Percentage of CoRSIVs for which target-capture bisulfite sequencing achieved various read depths; each point represents one of 807 samples. D Plots of read depth at two target regions illustrate specificity of targeting across all six tissues. The Y-axis scales are same for each region and indicated for thyroid. E Scatter plots between all possible tissue pairs illustrate high inter-tissue correlations at a CoRSIV within HPCAL1. F Heat map of inter-tissue correlations across 4086 CoRSIVs shows generally high correlation coefficients between all possible tissue pairs. G For the 232 tissue samples from 53 donors with data on at least 4 tissues (excluding cerebellum), unsupervised hierarchical clustering of methylation data at 2349 fully informative CoRSIVs groups perfectly by donor