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Table 1 Summary of single cell or subcellular spatial omics technologies. The table only includes single cell or subcellular resolution spatial omics technology, excluding spatial barcoding methods as it loses the organization context (e.g., CITE-seq, ZipSeq, ExSeq, XYZeq, and sci-space). MERSCOPE employs MERFISH as a platform solution. Slide-SeqV2 and DBiT-seq could be near single-cell. The plex numbers are based on the maximum validated or claimed in publications, as of March 2022. Ab-based Antibody-based methods (multiplexed spectrometry), mFISH Multiplexed FISH (probe-based methods), NGS Next-generation sequencing, SR Not specified and stated as subcellular resolution; falls within the capacity of resolution microscopy, WT Whole transcriptome

From: Spatial omics technologies at multimodal and single cell/subcellular level

Technology

Type

Target

Resolution (nm)

Year

Company

Plex

FISSEQ

NGS

RNA

600

2014

FISSEQ

8742

MIBI

Ab-based

RNA, protein

350

2014

IonPath

40

IMC

Ab-based

RNA, protein

1000

2014

Fluidigm

37

ChipCytometry

Ab-based

Protein

500

2019

Canopy

120

MERFISH

mFISH

RNA

100

2015

Vizgen

10,000

CyCIF

Ab-based

Protein

110

2015

Rarecyte

261

seqFISH+a

mFISH

RNA

100

2016

Spatial Genomics

10,000

STARmap

NGS

RNA

SR

2018

N/A

1020

Phenocycler (CODEX)

Ab-based

RNA, protein

260

2018

Akoya

100

osmFISH

mFISH

RNA

SR

2018

N/A

48

Slide-seqV2b

NGS

RNA

10,000

2020

N/A

WT

HDST

NGS

RNA

2000

2019

N/A

WT

Split-FISH

mFISH

RNA

SR

2020

N/A

317

HybISS

mFISH

RNA

SR

2020

N/A

124

DBiT-seq

NGS

RNA, Protein

10,000

2020

AtlasXomics

WT

Stereo-Seq

NGS

RNA

500

2021

BGI STOmics

WT

ExSeq

NGS

RNA

1000

2021

N/A

WT

Molecular Cartography

mFISH

RNA

200

2021

Resolve Biosciences

100

Cell DIVE

Ab-based

Protein

SR

2021

Cytiva

60

CosMxc

mFISH

RNA, protein

50

2022

NanoString Technologies

960

Xeniumc

mFISH

RNA, protein

50

2022

10X Genomics

1000

Visium HD

NGS

RNA

5000

2021

10X Genomics

WT

Esper

mFISH

RNA

260

2021

Rebus Biosystems

30

Seq-scope

NGS

RNA

600

2021

N/A

WT

PIXEL-seqc

NGS

RNA

1000

2021

N/A

WT

MOSAICA

mFISH

RNA

100

2022

N/A

60

MICS

Ab-based

Protein

100

2022

Miltenyi Biotec

100

EEL FISHc

mFISH

RNA

200

2022

Rebus Biosystems

440

coppaFISHc

mFISH

RNA

SR

2022

N/A

72

  1. aupdated version of seqFISH; bupdated version of Slide-seq
  2. cIn preprint or not publicly available yet