Table 1 All reagents used for sample preparation with nPOP
From: Exploring functional protein covariation across single cells using nPOP
| Â | Reagent | Dispense conc. | Volume added | Concentration in droplet |
|---|---|---|---|---|
1 | DMSO | 100 % | 8 nl | 100 % |
2 | Cell | 300 cell/μl in 1× PBS | 300 pl | 1 cell |
3 | Digest mix | Â | 13 nl | 60 % (aq) |
|  | Trypsin Gold (aq) | 150 ng/μl |  | 90 ng/μl |
| Â | HEPES (aq) | 6 mM | Â | 4 mM |
4 | TMT (DMSO) | 8.3 μg/μl | 30 nl | 8.3 μg/μl |
5 | mTRAQ (DMSO) | 8.3 μg/μl | 30 nl | 8.3 μg/μl |
6 | TEAB (aq)a | 200 mM | 20 nl | 50 mM |
7 | Hydroxylamine (HA) | 5 % | 2 × 20 nl | 3 % |