Fig. 5

Metabolic enzymes and ribosomal proteins covary and exhibit abundance gradients within the non-primed melanoma subpopulation. a Scatter plots of protein abundances across single cells for proteins identified to be differentially abundant between cluster A and B in the integrated data set of pSCoPE and plexDIA measurements. b Correlations between proteins computed within cluster A. Glycolytic proteins (which are upregulated in cluster A) are positively correlated to each other and inversely correlated to proteins participating in oxidative phosphorylation (which are upregulated in cluster B). Correlations are computed only from cells assigned to cluster A. Exploration of all differentially abundant proteins between cluster A and cluster B reveals a gradient of states for proteins upregulated in cluster A (c) and for proteins upregulated in cluster B (d)