Fig. 4

Proteomic, cell cycle, and metabolic differences between melanoma clusters. a Single melanoma cells projected by PCA and colored based on the mean abundance of proteins whose transcripts were previously identified as markers of primed cells by Emert et al. [7]. The single cells were also colored by the average abundance of protein sets exhibiting significant (FDR < 1%) enrichment in cluster A (bottom row) or cluster B (top row). b Distributions of cells by CDC phase for cells from clusters A and B. CDC phases were determined from marker proteins from Fig. 3. c Protein sets showing distinct covariation in clusters A and B. The analysis and display are as in Fig. 3e. d Mean reporter ion intensity versus cell diameter for melanoma cells. e The asterisk (∗) indicates p < 0.05. Protein concentrations for cells form cluster A and cluster B as defined by total reporter ion signal divided by cell volume. f Glycogen content measured from 10,000 cells from cluster A or cluster B. g Glycolytic enzymes have higher abundance in cluster A