Fig. 5

Effect of tumor purity and sequencing depth for SV detection. a Comparing small SV (50bp–30kb size range) detection sensitivities in different tumor purities in 5, 10, 20, 50, 75, and 100% tumor purity with 100x sequencing coverage. b Comparing large SV (>30kb) detection sensitivities in different tumor purities in 5, 10, 20, 50, 75, and 100% tumor purity at 100x sequencing coverage. The blue horizontal bars on the left show the total number of SVs in the specific tumor purity, pink bar with black dots denote number of SVs called in each tumor purity data. The top black vertical bars display the total concordance calls among the different tumor purities. c Line charts display SV detection sensitivity among different sequencing depth (10X, 30X, 50X, 100X, 200X, 300X) and different tumor purities (5%, 10%, 20%, 50%, 100%). The reference call set was built with consensus methodology and used the SVs from our consensus call set. The recalled SVs were separated into TRA (Translocation), DEL (Deletion), DUP (Duplication), and INV (Inversion)