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Fig. 2 | Genome Biology

Fig. 2

From: m6A RNA methylation impairs gene expression variability and reproductive thermotolerance in Arabidopsis

Fig. 2

AtALKBH10b is required for reproductive thermotolerance. a Density distribution of m6A in the wt and 10b-1 mutant flowers. UTR and CDS are marked as dark and light grey boxes, respectively. CS, non-stressed control sample. b Cumulative distribution of the m6A enrichment fold change in 10b-1 normalized against Col-0. P value was obtained by the one-tailed Wilcoxon rank sum test. c Cumulative distribution of expression variability difference between wt and 10b-1 determined for the 10B-regulated and randomly selected transcripts. The 10B-regulated genes are those with at least two-fold increase of m6A enrichment in 10b-1 compared to wt. P value was obtained by the one-tailed Wilcoxon rank sum test. d Heatmap of FC expression upon heat stress in different samples. Genes upregulated by the heat treatment in wt leaves by at least two-fold were selected. e Fraction of normal and short siliques of plants subjected to the control and heat stress treatments. CS, control sample; HS, heat-stressed sample. Siliques that are longer than 10 mm were considered normal and those shorter than 10 mm were counted as short siliques. f A representative image for siliques of the non-stressed and heat-stressed wt and 10b-1 plants. Bar is 10 mm. g Schematic illustration of m6A-associated gene expression variability. Bars represent the level of gene expression of individual samples in heat. Floral tissues exhibit stronger m6A RNA methylation and lower expression variability. Dashed line indicates a critical threshold required for heat tolerance. Reduced expression variability in flowers diminishes the gene activation to a level below the critical point

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