Fig. 5

Validations of allele-specific regulatory genomic regions for reGenes. a–c The genome browsers showed MOA-seq signals of reGenes between heat and control in B73 genome coordinates. MOA-seq genome coverage was normalized using the RPGC methods to make tracks comparable. a Zm00001d005114; b Zm00001d017187; c Zm00001d042183; d–f Expressions of assessed reGenes in genotypes associated with the detected reQTL as reference allele or alternative allele under control and heat condition. d Zm00001d005114; e Zm00001d0171873; f Zm00001d04218; g–h Raw read ratios of g Mo17- or h Oh43-specific allele to B73-specific allele of Zm00001d005114 or Zm00001d042183 in the hybrid MB or OB under control and heat condition (n=3). i–k Around 2kb putative promoter region per reGene was amplified separately from B73 genotypes and relative alternative genotypes for the dual luciferase assay (n=3). i Promoters amplified from Zm00001d005114 and Zm00014a020759. j Promoters amplified from Zm00001d017187 and Zm00035ab255850. k Promoters amplified from Zm00001d042183 and Zm00039ab143500