Fig. 3
From: Mapping responsive genomic elements to heat stress in a maize diversity panel
Detected responsive genes and eQTLs in the studied maize diversity panel. a Correlation of effect sizes of selected top significant cis-eQTLs versus identified responsive eQTLs (reQTLs). b Distribution of cis-eQTLs nearby annotated genes. Control cis-eQTLs represented cis-eQTLs identified using control expression data and heat cis-eQTLs represented cis-eQTLs identified using heat expression data. The y-axis indicated the proportion of each cis-eQTL class. TSS: transcription start sites; TTS: transcription terminal sites. c Log2 fold changes between heat and control for identified responsive genes (reGenes). The x-axis indicated log2 fold changes of expressions of genes carrying reference allele between heat and control. The y-axis indicated log2 fold changes of expressions of gene carrying alternative alleles between heat and control. The CPM of 1 was added to both denominator and numerator to the ratio to enable the calculation. Based on the ratio of median expression in heat to control, reGenes were subclassified into RefControl, RefHeat, AltControl, and AltHeat. d–g Four cases show different responsive genes. Associated SNP in each subpanel was indicated as “SChr_Pos.” For example, “S4_80278057” was the SNP located on 80,278,057 bp position of chromosome 4. h Connected curve indicates the significant p-value of interacted term between SNPs and targeted reGene (bold). The heatmap shows the pairwise correlation of SNPs in the displayed genomic region. Blue star indicates the top selected cis-eQTL for genome-wide reQTL identification analysis. i Same as the panel h, but showing gene Zm00001d030549