Fig. 4

Wastewater versus clinical abundance estimates for B.1.1.7 and B.1.526 in New Haven from early January 2021 to late April 2021. Dates of clinical sampling correspond to the date of specimen collection. In addition, ddPCR-based abundance estimates of lineages with the H69/V70 deletion (likely B.1.1.7) are shown for wastewater samples taken every six days. Confidence intervals are computed from ddPCR confidence intervals for measured copied of the H69/V70 deletion and measured copies of wild type present (see the “Methods” section). Vertical dashes on the x-axis indicate timepoints where wastewater sequencing data was obtained that passed the filtering criteria (Ct value < 31 or Ct < 34 with at least 0.5M reads aligned)