Fig. 2

Benchmarking results on simulated wastewater sequencing data of a representative mixture of background sequences and a variant lineage (VOC; here: B.1.1.7, B.1.351, B.1.427, B.1.429, P.1). a Estimated lineage abundance (VOC frequency) versus true abundance on whole genome sequencing data with a depth of 1000×. b Estimated lineage abundance (VOC frequency) versus true abundance on Spike-only sequencing data with a depth of 10,000×. c, d Relative prediction error per lineage for the estimated frequencies presented in panels a and b, respectively. Relative prediction errors are defined as the absolute difference between true and estimated frequency, relative to the true frequency. VOC, variant of concern