Fig. 2

Tissue specificity of pseudogene expression. a Tissue samples from mouse. Somatic tissues were pooled into one sample for library preparation for each sex. b Length distribution of transcripts in ENSEMBL annotation and PacBio sequencing. c Biotype of pseudogenes detected by Iso-seq. d Venn plot shows the overlap between Iso-seq detected pseudogenes and transcribed pseudogenes annotated by GENCODE. e Expression pattern of Iso-seq detected pseudogene 4933401B06Rik. f Expression level of Iso-seq detected pseudogenes and other pseudogenes. g Proportion of expressed pseudogenes under different thresholds. h Principal component analysis (PCA) of human pseudogenes. i PCA of 1:1 orthologous pseudogenes between human and mouse. j Proportion of human expressed pseudogenes with different evolutionary ages. k Tissue specificity of human pseudogene expression. l Distribution of the tissue with maximum expression level of human pseudogenes