Fig. 5

Predict the regulatory relationships affecting SOC TFs. a Enrichment of the Basenji model predicted key TF motifs in promoter sequences of TWAS significant genes of SOC. -log10 (FDR) represents the significant enrichment level. b Bar graph demonstrates the enrichment of the NAC motif in the promoter sequences of TWAS significant genes of SOC regulated by Hotspot87-88. -log10 (E-value) represents the significant enrichment level. c SOC phenotype of OE lines (BnaA07.NAC13, BnaC07.NAC13). Values are means (SD; n = 5 biological repeats). *P < 0.05 compared with WT and **P < 0.01 compared with WT in Student’s t test. d Representative confocal images of LDs (BODIPY 493/503 fluorescence, false color red) in tobacco leaves. The blue color shows chloroplast auto-fluorescence. Images were collected at the same magnification. Mock infection was infiltrated with media only; P19 was used as a viral suppressor of transgene silencing and was coexpressed in all BnaA09.SCL31 and BnaA07.NAC13 treatments. Bar = 20 μm. e–g Bar graph showing the relative LUC/REN ratio in the dual-luciferase assay. Values are means (SD; n = 3 biological repeats). BnaC03.SLP1(SHEWENELLA-LIKE PROTEIN PHOSPHATASE 1.h A model map predicting the regulatory mechanism of Hotspot87-88 in regulating SOC. The hexagonal shape shows the hotspot region, the circular shape represents the TFs, and the blue box inside represents the downstream TWAS significant genes of SOC