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Fig. 7 | Genome Biology

Fig. 7

From: New roles for AP-1/JUNB in cell cycle control and tumorigenic cell invasion via regulation of cyclin E1 and TGF-β2

Fig. 7

Overexpression of JUNB promotes tumor development and metastasis in vivo. JunB induces tumor growth in immunodeficient mice. UTA6-JUNB and UTA6-control cells were subcutaneously injected in NSG mice. Tumor size in the subcutaneous xenograft model was measured twice a week (lower panel) and 3 months after implantation, mice were euthanized and dissected. Photographs of tumors retrieved at the end of the experiment (upper panel). JunB induces tumor metastasis in immunodeficient mice. UTA6-control- and UTA6-JUNB-induced primary tumors and GFP expression in these tumors (Left panels, the scale bar is 1 mm), and representative image of liver and lung UTA6-JUNB-induced metastasis and GFP expression in these metastases (right panels, the scale bar is 1 cm). Arrows indicate metastatic lesions. Table reporting EGFP-expressing metastases in lung and liver of the 5 mice xenografted with UTA6-JUNB cells. C–E Higher expression of JUNB, CCNE1, and TGFB2 in UTA6-JUNB liver metastases than in primary UTA6-JUNB tumors. Representative immunofluorescence images of paraffin-embedded UTA6-JUNB and UTA6-Control primary tumor tissues, and UTA6-JUNB liver metastases. C Corresponds to JUNB, D corresponds to CCNE1, and E corresponds to TGFB2 analyses, respectively. DAPI staining (blue) indicates cell nuclei. Scale bars are indicated. JUNB and TGFB2 levels are increased in the border of UTA6-JUNB expressing primary tumors. Representative immunofluorescence images of the central and peripheral zones of UTA6-JUNB. JUNB, TGFB, CCNE1 (red), and cell nuclei (blue) are observed. JUNB and TGFB2 levels are similar in the central and peripheral zones in control tumor tissues. Representative immunofluorescence images of the central and peripheral zones of UTA6-Control tumor tissues. JUNB, TGFB (red), and cell nuclei (blue) are observed. JUNB and TGFB colocalization in the UTA6-JUNB primary tumor border. Representative immunofluorescence images of UTA6-JUNB primary tumor tissue. TGFB2 (magenta), JUNB (green), and cell nuclei (blue) are observed. Arrows indicate cells co-expressing JUNB and TGFB2 proteins. Integrin β1 and Fibronectin levels are increased in the border of UTA6-JUNB expressing primary tumors. Representative immunofluorescence images of UTA6-JUNB and control primary tumor tissues. Integrin β1 (red, left panel), fibronectin (red, right panel), and cell nuclei (blue) are shown. Scale bars are indicated. LT: Liver Tissue MT: Metastatic tissue

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