Fig. 3

Characterization of JUNB cistrome. A Proportional Venn diagram representing the intersection of JUNB binding sites identified in two independent ChIP-seq biological replicates. B Genomic distribution of JUNB binding sites. The pie chart shows the distribution of JunB binding sites across the genome. Intergenic refers to the regions located from >10 kb from one TTS (terminal transcription site) to >10 kb of the TSS of the closest gene in 3'. C Distribution of the distances of JunB peaks to the nearest transcription start site (TSS). D Heatmaps of ChIP-seq signals around JUNB peak centers display high H3K27ac and H3K4me1 occupancy. The heatmaps illustrate the JUNB ChIP-seq signals of U2OS in comparison to published ChIP-seq profiles of H3K4me3, H3K4me1, and H3K27ac of U2OS cells. Regions were sorted according to decreasing JUNB signal intensity (± 6kb around the centered summits). E Distribution of peaks associated with JUNB binding sites either at candidate active promoter (cAP), candidate active enhancer (cAE), candidate inactive promoters (cIP), candidate inactive enhancers (cIE), and regions that do not contain histone mark specifying active or inactive enhancers or promoters (Other) as defined in the text. F Top-enriched DNA motifs identified within JUNB ChIP-seq peaks using the MEME suite. The most enriched DNA motif corresponds to the TRE/AP-1 motif, and the less enriched DNA motif corresponds to the CRE motif. G Venn diagram representing the intersection of the number of genes up- or downregulated by siJunB (siJUNB-792 and siJUNB-848; transcriptomic analysis), and genes containing JUNB binding sites annotated to the closest TSS (ChIP-seq analysis) and associated at cAP, cAE, cIP, and cIE