Fig. 2

Binding of miR-181b-5p to the 3′UTR of the FNIP2 T allele. A TargetScanHuman predictions (http://www.targetscan.org/vert_72/) for rs2291007 genomic region. B rs2291007 frequencies across European (EUR) and African (AFR) populations (https://www.ensembl.org/). C Sfold (https://sfold.wadsworth.org/cgi-bin/index.pl) prediction for binding of miR-181b-5p to the rs2291007 region. D Dual-luciferase assays on psiCHECK vectors, for the binding of miR-181b-5p to rs2291007 region in presence of C or T alleles. Sequences for FNIP2 3′UTR with rs2291007 C or T alleles were cloned into psiCheck2 by ATUM. The relative luciferase activity (Renilla luminescence/firefly luminescence) was determined after transfection using the Dual-Luciferase Reporter assay system representing the translational repression of FNIP2 upon binding to their 3′UTR of miR-181b-5p. Unpaired Student’s t test was used