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Fig. 3 | Genome Biology

Fig. 3

From: scTAM-seq enables targeted high-confidence analysis of DNA methylation in single cells

Fig. 3

scTAM-seq captures the B-cell differentiation process in bone marrow. a Heatmap of single-cell DNAm values for 5340 cells and 313 amplicons in the digested bone marrow sample. b–d Visualization of DNAm data in low-dimensional space (UMAP), with labels inferred from bulk DNAm (b), across differentiation pseudotime (inferred with Monocle3, c), or with labels transferred from CITE-seq data (d). e Visualization of surface-protein expression for cells embedded in the DNAm UMAP. The value shows the CLR-normalized expression values (see “Methods”). f, g Relationship between DNAm and log-normalized gene expression across B-cell differentiation showing a positive (f, CXCR5, CpG located in promoter region) and negative correlation (g, SMARCA4, CpG located in intronic region), respectively

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