Fig. 1

ScTAM-seq identifies cellular subtypes in B cells from peripheral blood. a Overview of the scTAM-seq workflow. b Per amplicon comparison of fraction of cells with at least one sequencing read in the undigested and digested bone marrow sample. c False-negative rate (estimated on B-cell differentiation amplicons, n=424, undigested bone marrow control) and false-positive rate (estimated on the constitutively unmethylated amplicons, n=32, digested bone marrow sample) across all amplicons of the respective class. d Heatmap (binary distance, Ward’s method) of binarized, single-cell DNAm values of 9583 cells across 313 high-performance amplicons in the peripheral blood sample. e Comparison of pseudo-bulk and bulk DNAm for the 424 B-cell differentiation-related amplicons. r: Pearson’s correlation coefficient. The colors correspond to the DNAm clusters in d