Fig. 1
Experimental design to compute pioneer activity. A We induced FOXA1 or HNF4A across a 1000-fold dox range, measured binding, collected binding signals at a reproducible set of binding sites, and then extracted a dox50 for each site. B Maximal pioneer activity (index = 1) occurs when a TF’s average affinity in accessible regions is similar to that in inaccessible regions. Pioneer activity decreases (index < 1) as the affinity in inaccessible regions decreases, causing an increase in the dox50 measurement. C The number of total peaks for each TF across each dox induction level. D Replicate RPKM binding signal at two example genomic sites. E Replicate fitted lines at two example genomic sites. F Full distribution of dox50 values for each TF