Fig. 3
From: Local CpG density affects the trajectory and variance of age-associated DNA methylation changes
Local SNPs associate with altered CpG methylation trajectories. a Examples of slope-QTLs. Spaghetti plots and boxplots of 3 slope-QTL CpG-SNP pairs. Left, spaghetti plots of individual methylation trajectories separated by genotype. Thin lines represent individual methylation trajectories and thick lines the mean methylation trajectory for that genotype. Right, boxplots of slope separated by genotype. Lines = median; Box = 25th–75th percentile; whiskers = 1.5 × interquartile range from box. SNP genotypes are annotated relative to the forward strand. b Slope-QTL SNPs are located in close proximity to the CpGs they affect. Histogram of the distances between slope-QTL lead SNPs and the CpGs they are paired with. c Nearby CpGs are also affected by slope-QTL SNPs. Line plot of the effect sizes calculated for CpGs within − / + 1 Kb of slope-QTL CpGs using each slope-QTL’s lead SNPs. Plotted is the mean normalized effect size in 50 bp Windows. Bold lines show the mean effect size and dashed lines and shaded area show the 95% confidence intervals. The data are shown in red and the results of 1000 random permutations shown in black