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Fig. 1 | Genome Biology

Fig. 1

From: Enhanced mitochondrial DNA editing in mice using nuclear-exported TALE-linked deaminases and nucleases

Fig. 1

Improved cytosine base editing efficiency in mouse blastocysts. a Plasmid construct expressing DdCBE-NES. The amino acid sequence of the NES from the MVM NS2 protein is shown. b Base editing targets in the mitochondrial and nuclear genomes. C(G)-to-T(A) conversion targets are shown in red, and mismatches between mtDNA and nuclear DNA are shown in lowercase blue font. c–e Target C(G)-to-T(A) editing efficiencies in mouse blastocysts. The sequencing data were obtained from cultured blastocysts that developed after one-cell stage embryos were microinjected with mRNA encoding the DdCBE or DdCBE-NES. mtDNA and nuclear DNA amplicons were respectively analyzed for the MT-ND5 gene and a potential off-target site on chromosome 4 (c), the MT-TrnA gene and an identical target site on chromosome 5, and MT-Rnr2 and an identical target site on chromosome 6. For mitochondrial-specific amplicons, data points from DdCBE-injected blastocysts are shown as magenta and those from DdCBE-NES-injected blastocysts as green. For nuclear DNA-specific amplicons, purple indicates DdCBE and light blue DdCBE-NES. All data sets represented in the graphs were obtained from at least three biologically independent samples. The exact p-values are *0.0189 and **0.0045 for c, *0.0327 for d, and *0.0881 for e. (N ≥ 3; n.s., not significant, *p < 0.05, **p < 0.01, and ***p < 0.001 using Student’s two-tailed t-test)

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