Fig. 6

Depletion of Mus81 in the presence of aphidicolin exacerbates the bias towards specific sites of large aCNAs. A Western blot to indicate loss of Mus81 protein in RPE1 cells after siRNA for 48 h. Vinculin was used as a loading control. B Immunofluorescence image of RPE1 cell going through anaphase, with ultrafine bridge, as detected by replication protein A (RPA70). C Quantification of segregation error rates in RPE1 in combination treatments of siControl, siMus81 with either DMSO or aphidicolin. D Quantification of occurrence of ultrafine bridges in anaphase cells. E Quantification of rates of micronuclei from cells treated as indicated. F Summary of CNAs identified in RPE1 cells after siRNA depletion of Mus81, combined with either DMSO or aphidicolin. G Rate of large CNAs occurring on chromosomes 1, 2, and 7 in RPE1 cells (in aphidicolin, or in aphidicolin after depletion of Mus81). H Summary of genomic features linked to aCNAs and hCNAs identified in this study