Fig. 2

Genomic features of aCNAs detected by single-cell whole genome sequencing. A Diagrams summarizing all RPE1 CNAs induced by indicated treatments, after removing clonal events (see “Methods”). Yellow lines indicate location of centromeres, purple lines represent RPE1-specific CFS locations. B Frequency of CNAs occurring in RPE1 cells after treatments as indicated (n = 90, 332, or 170 cells, respectively). C Distribution of CNAs divided by size and by gains versus losses in RPE1 (31 CNAs identified in DMSO, 136 in aphidicolin, 118 in HU). D Rate of CNA classes per cell (small CNAs defined as less than 20 Mb; large defined as 20 Mb or larger). E Frequencies of CNAs (left y-axis) across each chromosome after aphidicolin or HU treatment as indicated, divided into large (>20 Mb) and small (< 20 Mb) CNAs. Dotted line indicates size of each chromosome (right y-axis)