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Fig. 2 | Genome Biology

Fig. 2

From: CRISPR-KRISPR: a method to identify on-target and random insertion of donor DNAs and their characterization in knock-in mice

Fig. 2

Assessment of in vivo OT indels introduced with Mmp9-Cr1 gRNA. A Schematic illustrating OT candidate sites (OCSs) identification and their confirmation procedure. OCSs for Mmp9-Cr1 gRNA were identified by CRISPR-KRISPR and by Cas-OFFinder. The fragments containing OCSs selected were PCR amplified from founder and wildtype (WT) mice. Amplicon sequencing of these fragments were performed using a high-throughput sequencer. B Manhattan plot of 802 cleavage sites detected by CRISPR-KRISPR. The length of bars represents CRISPR-KRISPR read count. The Mmp9-Cr1 on-target site is indicated in red. Top 47 OCSs are indicated in blue. C The top 47 OCSs detected by CRISPR-KRISPR and three predicted OCSs by Cas-OFFinder are shown. D Sequence logo of cleavage sites detected by CRISPR-KRISPR. E Venn diagram showing the number of OCSs and overlap between those OCSs predicted by Cas-OFFinder and CRISPR-KRISPR

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