Fig. 2

Implementation of the optimised INSERTseq protocol. a Overview of the library prep process. Genomic DNA is extracted, fragmented, end-repaired and A-tailed followed by ligation of an adaptor that contains an UMI for read clustering and a barcode for sample demultiplexing in the computational pipeline. b Schematic representation of sequenced reads structure. c Overview of the analysis pipeline. Reads are clustered by UMI, integration sequence and adapters are filtered and trimmed, reads are mapped against the reference genome and significant peaks are reported and annotated. d Exo I endonuclease effect on fraction of enriched reads, represented as fraction of LTR enriched reads (blue) over non-enriched. e Comparison of count distribution for each insertion with (purple) or without (yellow) UMI clustering in MOPO sample and “100 PB clones” sample. Top panel shows the distribution of the number of reads per insertion in logarithmic scale. Bottom panel shows the interquartile range of the distribution