Fig. 6

MBD6 is essential for BAP1-dependent gene expression in SCLC. The average plot (A) and representative tracks (B) show the global reduction of BAP1 in MBD6-depleted SCLC cells by two distinct sgRNAs. C Gene Set Enrichment Analysis (GSEA) of BAP1 gene signature enrichment in MBD6-depleted conditions. D The log2 fold-change heatmap shows the reduction of BAP1 peaks at MBD6 loci in cells transduced with two distinct MBD6-specific sgRNAs. E The log2 fold-change heatmap shows the change of expression levels of genes nearest to MBD6 peaks after MBD6 (left two lanes) and BAP1 (right two lanes) depletion by CRISPR, n=2. F NCI-H1963 cells were transduced with either non-targeting sgRNA or two distinct sgRNA specific to ASXL1/2/3 or BAP1. The protein levels of BAP1, ASXL1, ASXL2, and ASXL3 were determined by western blot. Tubulin was used as an internal control, n = 2. G The log2 fold-change gene expression heatmap shows BAP1/MBD6 co-targeted genes in ASXL1/2/3-depleted conditions by CRISPR knockout, n=2. H Pathway analysis by Metascape of genes that are commonly downregulated upon BAP1, MBD6, or ASXL3 depletion in NCI-H1963 cells. I The mRNA levels of NEUROG2 and CDK18 were determined by real-time qPCR in NCI-H1963 cells transduced with either non-targeting sgRNA, two different BAP1-specific sgRNAs, or two different ASXL1/2/3 sgRNAs