Fig. 5

Characterization of MBD6 occupancy in SCLC cells. A The representative tracks show chromatin occupancy of MBD6, ASXL1/2/3, and BAP1 binding sites in the human SCLC cell line NCI-H1963. B The bar plot shows the feature distribution of MBD6, ASXL1/2/3, and BAP1 peaks in NCI-H1963 cells. C Sorted and centered heatmaps generated from ChIP-seq and ATAC-seq data analyses show the occupancy of MBD6, ASXL1/2/3, BAP1, H3K27Ac, H3K4me1, H3K4me3, and H3K27me3 in NCI-H1963 cells. All signals were centered on MBD6 peaks, which were classified into three clusters, based on k-means clustering according to MBD6, H3K4me1, H3K4me3, and H3K27Ac ChIP-seq. D Representative tracks showing the occupancy MBD6, BAP1, ASXL1-3, and histone marks. E Pathway analysis was performed using ChIPseeker with genes nearest to MBD6 three cluster peaks defined in C. F Motif enrichment analysis of MBD6 three clusters’ peaks defined in C from NCI-H1963 cells. G The box plot shows the log2-fold-change of H3K4me1, H3K4me3, and H3K27Ac signals versus input at MBD6/BAP1/ASXL1, MBD6/BAP1/ASXL2, or MBD6/BAP1/ASXL3 co-occupied genomic regions