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Fig. 1 | Genome Biology

Fig. 1

From: H3K18 lactylation marks tissue-specific active enhancers

Fig. 1

H3K18la marks active CGI promoters. A Western blots showing H3K18la and H3 protein expression in all included samples (n = 3). The arrows indicate 15 kDa. B MDS of active hPTMs profiled from various mouse samples, quantified over 3000 bp genome-wide tiles. C Correlation heatmap of genome-wide, quantified H3K18la peak levels (biological replicates (n = 2) for mESC-ser, mESC-2i, ADIPO, GAS, PIM, and MT. For MB and BMDM; n = 1. Pearson’s correlation coefficient R is displayed as color gradient. D Distribution of H3K18la peaks of all mouse samples across genomic features. E H3K18la peak fold enrichment adjusted for genome-wide feature size for all mouse samples. F Venn diagrams depicting the promoter overlaps marked by the active hPTMs in mESC-ser, GAS, and PIM samples. Overlaps are colored according to the absolute number of promoters marked by various combinations of active hPTMs. Percentages indicate the fraction of actively marked promoters belonging to each group. G Scatterplots showing pairwise correlation of promoter H3K18la levels with other hPTM levels (log2CPM) highlighting the promoters of genes with highest (red, n = 2000) or lowest (cyan, n = 2000) normalized gene expression (RPKM) for mESC-ser, GAS, and PIM. Pearson’s correlation coefficient R and p-values are indicated. H Normalized gene expression (log2RPKM) per gene category is shown as boxplots. Gene categories are defined by the combination of active hPTMs as F. I Scatter plots showing the correlation between promoter H3K18la levels (log2CPM, y-axis) and expression of the corresponding gene (log2RPKM, x-axis) for mESC-ser, GAS, and PIM. Spearman’s correlation coefficient R and p-values are indicated

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