Fig. 1

Performance evaluation of MntJULiP on simulated and real data. A Comparative evaluation of several methods on 25 control and 25 perturbed simulated RNA-seq data sets. B Venn diagram of methods’ gene-level DSR predictions on 24 healthy and 20 epileptic mice. C Differential splicing at the Zxdc gene locus discovered in the mouse hippocampus data by MntJULiP (DSA); no two introns share an endpoint; therefore, the gene could not have been discovered by other tools. Introns are annotated with the fold change values in the comparison of healthy and epileptic mice. D Venn diagram of DSR genes, and heatmap of DSR introns discovered with MntJULiP in a multi-way comparison of cerebellum, cortex, and lung GTEx RNA-seq samples. Rows were clustered using the Ward distance. E Distribution of program-predicted features by number of comparisons for three methods: (i) union of MntJULiP predicted features from all (21 total) pairwise comparisons, (ii) MntJULiP multi-way predicted features, and (iii) union of LeafCutter predicted features from all (21) pairwise comparisons. F Heatmap of DSR introns discovered from the multi-way comparison of 7-stage taste organoid RNA-seq data. Heatmaps show PSI values of differentially spliced introns. Clustering was performed with the Bray-Curtis distance and simple averaging