Fig. 6

Genome-wide mosaicism persists to the bovine blastocyst stage (Full overview in Additional file 9: Fig. S9). A Experimental setup. IVM: in vitro maturation; IVF: in vitro fertilization. B Circos plots of two bovine blastocysts in which each circle represents the genome constitution per chromosome of a single cell. In E28, cell 1 presented with alternating biparental disomic and maternal chromosomes while the remaining cells were androgenetic. In E29, cell 1 was found to be androgenetic while cells 2–5 and 7–11 were biparental diploid. Cell 6 displayed alternating paternal chromosomes, maternal chromosomes, or nullisomies. C A visual interpretation of the paternal haplarithm profiles (Additional file 9: Fig. S9) of two cells from each embryo. For each blastomere from top to bottom, respectively, we depict the area and copy number inherited from paternal homolog 1 (H1, light blue) or paternal homolog 2 (H2, dark blue), together forming the paternal haplotype. Shifts from H1 to H2 represent recombination sites. Simultaneous presence of H1 and H2 represent regions of heterodisomy. In E28, identical homologous recombination sites were retrieved in the androgenetic cells and biparental disomic regions of cell 1 (black arrows). Heterodisomic regions throughout the genome of cells 2–9 (Pat-BAF = 0.5) revealed the presence of a second paternal haplotype. In E29, the androgenetic and diploid cells present with different paternal homologous recombination sites (green arrows). Copy number is not represented in the androgenetic cell 1 of E29 since the value is normalized to a relative value of two in the absence of chromosomal errors or a second parental genotype. Segmental chromosomal errors are not depicted