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Fig. 2 | Genome Biology

Fig. 2

From: Parental genomes segregate into distinct blastomeres during multipolar zygotic divisions leading to mixoploid and chimeric blastocysts

Fig. 2

Study design and data analysis by haplarithmisis. A Study design. IVM: in vitro maturation; IVF: in vitro fertilization; WGA: whole-genome amplification; e=number of embryos; b=number of blastomeres and fragments. B Haplarithm patterns for a selection of genomic constitutions per chromosome (e.g., normal disomy, paternal monosomy, and paternal meiotic/dispermic uniparental heterodisomy (Full overview in Additional file 2: Fig. S2, A). Corresponding GW profiles (e.g., biparental diploid or androgenetic) are characterized by the manifestation of those patterns throughout the (majority of the) genome. During initial parental phasing, single-cell B-allele frequency (BAF) values are assigned to parental informative SNPs, rendering two paternal and two maternal subcategories (blue and red lines). Defined single-cell BAF values of the segmented subcategories in the sample form haplotype blocks, demarcated by pairwise breakpoints, i.e., homologous recombinations. Haplotype blocks, as well as the distance between the parental SNP subcategories in the paternal and maternal haplarithm, respectively, and the positioning of homologous recombinations, denote the origin and nature of copy number (more detailed explanation in Additional file 2: Fig. S2 and [64])

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