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Fig. 6 | Genome Biology

Fig. 6

From: Control of immediate early gene expression by CPEB4-repressor complex-mediated mRNA degradation

Fig. 6

CPEB4-dependent IEG mRNA degradation. A Decay curves of eight IEG mRNAs containing CPEB4 binding sites were quantified in parental HeLa cells, control clone #2 and CPEB4 KO clones #44 and #45. Transcription was terminated by treatment of cells with 5 μg/ml actinomycin D, and total RNA was extracted after 0, 2, and 4 h. The mRNA levels were measured by qRT-PCR; 18S rRNA was used for normalization. Data are presented as mean ± SD (n = 3). B The same analysis as in A was conducted for four non-IEG mRNAs. C Schematic illustration of the experimental workflow used to measure IEG mRNA half-lives following mitogenic stimulation. HeLa cells were seeded at day 0 and serum-starved for 24 h before stimulation with 20 ng/ml human epidermal growth factor (EGF). Following 2 h of stimulation, mRNA stability was measured as described in A. D Decay curves of IEG mRNAs JUNB, FOS, and the non-IEG mRNA KIAA0232 were quantified following stimulation of HeLa cell lines with EGF as described in A and C. Data are presented as mean ± SD (n = 5)

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