Fig. 4

Comparative CPEB1-4 complex composition. A BioID-detected changes in the CPEB1(6A) proximome between PI (blue) and MII (yellow) (n = 3). The preys are compared in terms of their enrichment rank. Note that these ranks admit draws. B CPEB1-4 proximomes in X. laevis PI oocytes, determined by BioID (n = 4). Hits include proteins enriched in N-terminal and C-terminal BirA fusions, relative to BirA alone. Hits meet the following criteria: they either have a positive fold change and P-adj < 0.05 relative to BirA alone or have 3 to 4 missing values in the control against 1 or none in the condition and a high relative abundance (greater than 25.th percentile). C STRING network plot of the CPEB proximome in Xenopodinae displaying high confidence interactions (score > 0.7) and only networks with more than one interaction. CPEBs have been highlighted in yellow. D Co-immunoprecipitation of endogenous preys with HA-tagged CPEBs confirmed by Western blot using specific antibodies (n = 3)